Literaturdatenbank
Literaturdatenbank |
|
 |
|
Johnson, A. J., Pessier, A. P., & Jacobson, E. R. (2007). Experimental transmission and induction of ranaviral disease in western ornate box turtles (terrapene ornata ornata) and red-eared sliders (trachemys scripta elegans). Veterinary Pathology, 44(3), 285–297.
Added by: Admin (14 Aug 2008 20:32:47 UTC) Last edited by: Beate Pfau (28 Mar 2009 10:10:36 UTC) |
| Resource type: Journal Article BibTeX citation key: Johnson2007b View all bibliographic details  |
Categories: General Keywords: Chelonia, Cheloniidae, Emydidae, Schildkröten = turtles + tortoises, Terrapene, Terrapene ornata, Trachemys, Trachemys ornata, Trachemys scripta, Trachemys terrapen, Veterinärmedizin = veterinary medicine, Viren = viruses Creators: Jacobson, Johnson, Pessier Collection: Veterinary Pathology |
Views: 2/682
Views index: 12% Popularity index: 3% |
| Abstract |
|
An experimental transmission study was designed to determine whether a causal relationship exists between a Ranavirus (BSTRV) isolated from a Burmese star tortoise that died and the lesions observed in that tortoise. A pilot study was performed with 3 box turtles (Terrapene ornata ornata) and 3 red-eared sliders (RESs; Trachemys scripta elegans) to assess their suitability in a larger study. Based on the outcome of this study, RESs were selected, and 2 groups of 4 RESs received either an oral (PO) or intramuscular (IM) inoculum containing10(5) 50% Tissue Culture Infecting Dose (TCID(50)) of a BSTRV-infected cell lysate. One turtle each was mock inoculated PO or IM with the same volume of uninfected cell lysate. Three of four IM-inoculated RESs developed clinical signs (nasal and ocular discharge , oral plaques , conjunctivitis and hyphema and extreme lethargy ). A Ranavirus was isolated from kidney homogenates of 3 euthanatized turtles; DNA sequences of a portion of the major capsid protein gene were amplified by polymerase chain reaction. Consistent histologic lesions were observed only in IM-inoculated turtles and included fibrinoid vasculitis centered on splenic ellipsoids, multifocal hepatic necrosis, and multicentric fibrin thrombi in a variety of locations, including hepatic sinusoids, glomerular capillary loops, and pulmonary capillaries. Virions compatible with Ranavirus were observed within necrotic cells of the spleen of 1 IM-inoculated turtle using transmission electron microscopy. This study fulfills Koch's postulates, confirming a causal relationship between BSTRV and the clinical and histologic changes in chelonians infected with this virus.
Added by: Admin Last edited by: Beate Pfau |