Literaturdatenbank
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Kundu, S., Das, K. C., & Ghosh, S. K. (2013). Taxonomic rank of indian tortoise: Revisit with dna barcoding perspective. In DNA Barcodes Vol. Band 1, (pp. 39–45).
Added by: Admin (06 Jan 2014 18:24:46 UTC) |
| Resource type: Book Article DOI: 10.2478/dna-2013-0003 ID no. (ISBN etc.): ISSN (Online) 2299-1077 BibTeX citation key: Kundu2013 View all bibliographic details  |
Categories: General Keywords: Genetik - genetics, Indotestudo elongata, Indotestudo forstenii, Manouria emys, Schildkröten - turtles + tortoises, Südasien - Southern Asia, Systematik - taxonomy, Testudinidae Creators: Das, Ghosh, Kundu Collection: DNA Barcodes |
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| Abstract |
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Testudinidae Taxonomic rank of terrestrial tortoise inhabiting in India and adjacent countries has been an unresolved issue. Wild living species of Manouria and Indotestudo genera have been classified through conventional taxonomy, but not adequately discriminated by mitochondrial cytochrome b gene. Cytochrome oxidase (COI) marker has been quite successful to achieve the exact species level information. There needs of an accurate sequence based effort for the extant northeast (NE) Indian tortoises to identify them accurately and to provide locality information. To estimate the Kimura-2-Parameter (K2P) divergences and to construct the Neighbour- Joining (NJ) phylogeny, we generated six partial COI sequences derived from expert-identified tortoise specimens. Both BLASTn and Bold-IDs revealed the definitive identity of Manouria, whereas the congeners of Indotestudo remain inconclusive by only a two-fold interspecific divergence gap with the other named Indian tortoise species. The NJ phylogeny readily differentiated the three Indian tortoise genera with their respective conspecies but depicted a nonsynonomous group of Indotestudo congeners. This study shows that the molecular identification of Manouria emys phayrei, Indotestudo elongata and an allopatric population of Indotestudo forstenii in NE India corresponds correctly with existing morphological key support.
Added by: Admin |