Literaturdatenbank
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Knotková, Z., Doubek, J., Knotek, Z., & Háiková, P. (2002). Blood cell morphology and plasma biochemistry in russian tortoises (agrionemys horsfieldi). Acta Veterinaria Brno, 71, 191–198.
Added by: Admin (14 Aug 2008 20:36:24 UTC) |
| Resource type: Journal Article BibTeX citation key: Knotkova2002 View all bibliographic details  |
Categories: General Keywords: Blut = blood, Schildkröten = turtles + tortoises, Testudinidae, Testudo, Testudo horsfieldii, Veterinärmedizin = veterinary medicine Creators: Doubek, Háiková, Knotek, Knotková Collection: Acta Veterinaria Brno |
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| Abstract |
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Testudo horsfieldii Testudinidae Morphological characteristics of peripheral blood cells and plasma biochemistry profile were examined in twenty clinically healthy Russian tortoises (Agrionemys horsfieldi). The blood samples were collected from dorsal coccygeal vein. The Pappenheim staining method was used as the basal method for the classification of the blood cells. For enriching staining panel the commercial kits Rapidiffâ , Lysocolorâ , Lymphocolorâ , Monocolorâ , Granulocolorâ and Neutrocolorâ were used. Ten different types of blood cells were determined: erythrocytes, thrombocytes, lymphocytes, monocytes, type-I cells (heterophils), type-II cells (eosinophils), type-III cells (azurophils), type-IV cells (basophils), type-Ia cells (toxic heterophils) and type-V cells (polychromatophil erythrocytes). Stained with Lysocolorâ , the positive reaction emerged in type-IV cells and less in type-III cells. Granulocolorâ stained positively type-I, Ia and II cells. General purpose stain Rapidiffâ was less vivid than the classical Pappenheim method. The differential count of leukocytes in the peripheral blood of clinically healthy tortoises was determined as follows: 0.372 ± 0.089 heterophils, 0.048 ± 0.011 eosinophils, 0.051 ± 0.020 azurophils, 0.050 ± 0.009 basophils, 0.467 ± 0.125 lymphocytes and 0.012 ± 0.006 monocytes. Plasma concentrations of total protein, glucose, uric acid, cholesterol, natrium, potassium, calcium, phosphorus and activities of ALP, ALT, AST were: 45.1± 6.8 g/l, 11.4 ± 1.3 mmol/l, 95.0 ± 20.1 m mol/l, 4.0 ± 1.7 mmol/l, 143.0 ± 6.5 mmol/l, 3.8 ± 0.9 mmol/l, 2.5 ± 0.9 mmol/l, 1.4 ± 0.4 mmol/l, and 4.5 ± 2.0 m kat/l, 0.8 ± 0.4 m kat/l, 1.1 ± 0.3 m kat/l, respectively. The differential count of leukocytes and biochemical values may be used as a standard profile for healthy Russian tortoises kept in captivity.
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