Literaturdatenbank |
Johnston, E. E., Rand, M. S., & Zweifel, S. G. (2006). Detection of multiple paternity and sperm storage in a captive colony of the central asian tortoise, testudo horsfieldii. Canadian Journal of Zoology, 84(4), 520–526.
Added by: Admin (14 Aug 2008 20:31:52 UTC) Last edited by: Beate Pfau (01 Nov 2008 17:22:56 UTC) |
Resource type: Journal Article BibTeX citation key: Johnston2006a View all bibliographic details |
Categories: General Keywords: Emydidae, Fortpflanzung = reproduction, Genetik = genetics, Glyptemys, Glyptemys muhlenbergii, Haltung = husbandry, Schildkröten = turtles + tortoises, Testudinidae, Testudo, Testudo horsfieldii Creators: Johnston, Rand, Zweifel Collection: Canadian Journal of Zoology |
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Abstract |
Testudinidae Reproductive success is a critical measure of an organism's fitness. Determining reproductive success in vertebrates is confounded by the concealed mechanism and timing of fertilization (e.g., sperm competition and storage). To assess the relationship between observed mating behavior and reproductive success in the central Asian tortoise, Testudo horsfieldii Gray, 1844, we determined individual genotypes from a captive colony of adults and their offspring. We constructed a size-selected genomic library from T. horsfieldii and screened for polymorphic microsatellite markers. The screen resulted in identification of two novel microsatellite regions. Cross-species amplification of microsatellite markers using primers developed for the bog turtle, Glyptemys muhlenbergii (Schoepff, 1801), resulted in isolation of three additional polymorphic microsatellites for T. horsfieldii. The five loci, which have between 5 and 17 alleles and observed heterozygosities between 0.44 and 0.90, were used to determine the frequency of multiple paternity in the captive colony. We found evidence for multiple paternity in 27% of the clutches examined, as well as evidence for overwinter sperm storage and variance in adult male reproductive success. These data indicate that ample opportunity exists for sperm competition and female mate choice in T. horsfieldii.
Added by: Admin Last edited by: Beate Pfau |